Coding

Part:BBa_K209474

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)

AarI C-D part, 3xSTOP-FKBP

Stop codons, flanked by AarI CD sites.


Usage and Biology

The purpose of this part is to insert stop codons in 3 different reading frames into the C-D position of an AarI fusion contruct. The FKBP domain comes after the stop codons, and is only included to facilitate extraction and ligation of this part.

Documentation and characterization

This construct was used to express the DOR receptor without a C-terminal fusion, described here:

http://2009.igem.org/Team:UCSF/NavigationPart2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None